Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing residual amounts of antibacterial agents as determined by chemical assay after multiple standard washings. Plug and sterilize at kPa 15 psi for 15 minutes.
The test is followed in the cases where the antibacterial agent is migratory i. Therefore, every necessary and 5. Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment.
Report of results will include an observation of zones of inhibition and growth under the specimen if present. Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width from about 8 mm to 4 mm wide.
Antimicrobial – Parallel Streak Method AATCC 147
Parallel Streak Method Developed in by AATCC Committee RA31; reaffirmed, ; editorially revised,; revisedwith title change; editorially revised and reaffirmed AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests.
This test method is subject to revision at any time by the responsible technical committee and must be reviewed every five years and if not revised, either reaffirmed or withdrawn. Neither the electronic file nor the hard copy print may be reproduced in any way.
The method is useful for obtaining a rough estimate of activity in that the growth of the inoculum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degrees of sensitivity.
Department of Health and Human services publication Biosafety in Microbiological and Biomedical Laboratories should be consulted see After incubation, a clear rest of interrupted growth underneath and along the sides of the test material mrthod antibacterial activity of the specimen.
Mmethod the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized specimen. Some of the bacteria used in this test are pathogenic; i. Mix well using appropriate agitation. At the conclusion of two weeks, make a fresh transplant from stock culture.
Users of this test method are expressly advised that determination of the validity of any such mthod rights, and the risk of infringement of such rights, are entirely their own responsibility. To constitute acceptable antibacterial activity, there must be no bacterial colonies directly under the sample aatc the contact area. Wear protective clothing and respiratory protection that prevents penetration by the bacteria.
AATCC takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this test method. The Parallel Streak Method has proven effective over a number of years of use in providing evidence of antibacterial activity against both Gram positive and Gram negative bacteria.
Purpose and Scope cluding corresponding untreated controls of the same material, are placed in intimate contact with nutrient agar see 7. Your comments will receive careful consideration at a meeting of the responsible technical committee, which you may attend.
AATCC Test Method
Peptone Bacto-peptone see Allow agar to gel firmly before inoculating. If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. Aaatcc specimens cut 25 – 50 mm They can be any convenient size. The precautions are ancillary to the testing procedures and are not intended to be all inclusive. Other recommended strains are listed below in Section 6. Leonard, Technical Director, at the address shown below.
The criterion for passing the test must be agreed upon by the interested parties. BoxManassas VA ; tel: Specimens of the test material, including corresponding untreated controls of the same material, are placed in intimate contact with growth agar which has been previously streaked with test organism. Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.
After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Other organisms can also be used depending on the intended end-use.
AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.
A standard strain of bacteria is used which is specific to the requirements of the material under test. Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity.
Uses and Limitations 1. This is not necessary if prepared, methdo medium is used. Precision and Bias 7. Antibacterial Activity Assessment of Textile Materials: May be sterilized in 1, mL borosilicate glass flasks and petri dishes poured from this. Individual reprints single or multiple copies of this method may be obtained by aatc AATCC at the above address or tel: A 50 TM Copyright?
Until a precision statement is generated for this test method, use standard statistical techniques in making any comparisons of test results for either within-laboratory or between-laboratory averages. Avoid contamination by using good sterile technique in plating and transferring. All OSHA standards and rules must also be consulted and followed. Adjust to pH 6. Avoid mutation by strict adherence to monthly stock transfers. Your comments are invited either for revision of this test method aatdc for additional methods and should be addressed to the AATCC Technical Center.
They may be any convenient size. Take care not to break the surface of the agar while making the streaks. Foreword The Parallel Streak Method has filled a need for a relatively quick methood easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.